A novel coronavirus associated with severe acute respiratory syndrome
Identifieur interne : 006421 ( Main/Exploration ); précédent : 006420; suivant : 006422A novel coronavirus associated with severe acute respiratory syndrome
Auteurs : Thomas G. Ksiazek [États-Unis] ; Dean Erdman [États-Unis] ; Cynthia S. Goldsmith [États-Unis] ; Sherif R. Zaki [États-Unis] ; Teresa Peret [États-Unis] ; Shannon Emery [États-Unis] ; SUXIANG TONG [États-Unis] ; Carlo Urbani [Viêt Nam] ; James A. Comer [États-Unis] ; WILINA LIM [Hong Kong] ; Pierre E. Rollin [États-Unis] ; Scott F. Dowell [Thaïlande] ; Ai-Ee Ling [Singapour] ; Charles D. Humphrey [États-Unis] ; Wun-Ju Shieh [États-Unis] ; Jeannette Guarner [États-Unis] ; Christopher D. Paddock [États-Unis] ; Paul Rota [États-Unis] ; Barry Fields [États-Unis] ; Joseph Derisi [États-Unis] ; Jyh-Yuan Yang [Taïwan] ; Nancy Cox [États-Unis] ; James M. Hughes [États-Unis] ; James W. Leduc [États-Unis] ; William J. Bellini [États-Unis] ; Larry J. Anderson [États-Unis]Source :
- The New England journal of medicine [ 0028-4793 ] ; 2003.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
Abstract
BACKGROUND A worldwide outbreak of severe acute respiratory syndrome (SARS) has been associated with exposures originating from a single ill health care worker from Guangdong Province, China. We conducted studies to identify the etiologic agent of this outbreak. METHODS We received clinical specimens from patients in seven countries and tested them, using virus-isolation techniques, electron-microscopical and histologic studies, and molecular and serologic assays, in an attempt to identify a wide range of potential pathogens. RESULTS None of the previously described respiratory pathogens were consistently identified. However, a novel coronavirus was isolated from patients who met the case definition of SARS. Cytopathological features were noted in Vero E6 cells inoculated with a throatswab specimen. Electron-microscopical examination revealed ultrastructural features characteristic ofcoronaviruses. Immunohistochemical and immunofluorescence staining revealed reactivity with group I coronavirus polyclonal antibodies. Consensus coronavirus primers designed to amplify a fragment ofthe polymerase gene by reverse transcription-polymerase chain reaction (RT-PCR) were used to obtain a sequence that clearly identified the isolate as a unique coronavirus only distantly related to previously sequenced coronaviruses. With specific diagnostic RT-PCR primers we identified several identical nucleotide sequences in 12 patients from several locations, a finding consistent with a point-source outbreak. Indirect fluorescence antibody tests and enzyme-linked immunosorbent assays made with the new isolate have been used to demonstrate a virus-specific serologic response. This virus may never before have circulated in the U.S. population. CONCLUSIONS A novel coronavirus is associated with this outbreak, and the evidence indicates that this virus has an etiologic role in SARS. Because ofthe death ofDr. Carlo Urbani, we propose that our first isolate be named the Urbani strain of SARS-associated coronavirus.
Affiliations:
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Ksiazek</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Special Pathogens Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Erdman, Dean" sort="Erdman, Dean" uniqKey="Erdman D" first="Dean" last="Erdman">Dean Erdman</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Respiratory and Enteric Virus Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>18 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Goldsmith, Cynthia S" sort="Goldsmith, Cynthia S" uniqKey="Goldsmith C" first="Cynthia S." last="Goldsmith">Cynthia S. 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Dowell</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>International Emerging Infectious Diseases Program</s1><s2>Bangkok</s2><s3>THA</s3><sZ>12 aut.</sZ></inist:fA14><country>Thaïlande</country><wicri:noRegion>International Emerging Infectious Diseases Program</wicri:noRegion></affiliation></author><author><name sortKey="Ling, Ai Ee" sort="Ling, Ai Ee" uniqKey="Ling A" first="Ai-Ee" last="Ling">Ai-Ee Ling</name><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Pathology, Singapore General Hospital</s1><s3>SGP</s3><sZ>13 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Department of Pathology, Singapore General Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Humphrey, Charles D" sort="Humphrey, Charles D" uniqKey="Humphrey C" first="Charles D." last="Humphrey">Charles D. 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Paddock</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Infectious Disease Pathology Activity, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Rota, Paul" sort="Rota, Paul" uniqKey="Rota P" first="Paul" last="Rota">Paul Rota</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Respiratory and Enteric Virus Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>18 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Fields, Barry" sort="Fields, Barry" uniqKey="Fields B" first="Barry" last="Fields">Barry Fields</name><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>19 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Derisi, Joseph" sort="Derisi, Joseph" uniqKey="Derisi J" first="Joseph" last="Derisi">Joseph Derisi</name><affiliation wicri:level="3"><inist:fA14 i1="09"><s1>University of California</s1><s2>San Francisco</s2><s3>USA</s3><sZ>20 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><settlement type="city">San Francisco</settlement><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Yang, Jyh Yuan" sort="Yang, Jyh Yuan" uniqKey="Yang J" first="Jyh-Yuan" last="Yang">Jyh-Yuan Yang</name><affiliation wicri:level="1"><inist:fA14 i1="10"><s1>Center for Disease Control, Department of Health</s1><s2>Taipei</s2><s3>TWN</s3><sZ>21 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Cox, Nancy" sort="Cox, Nancy" uniqKey="Cox N" first="Nancy" last="Cox">Nancy Cox</name><affiliation wicri:level="1"><inist:fA14 i1="11"><s1>Influenza Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>22 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Hughes, James M" sort="Hughes, James M" uniqKey="Hughes J" first="James M." last="Hughes">James M. Hughes</name><affiliation wicri:level="1"><inist:fA14 i1="12"><s1>National Center for Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>23 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Leduc, James W" sort="Leduc, James W" uniqKey="Leduc J" first="James W." last="Leduc">James W. Leduc</name><affiliation wicri:level="1"><inist:fA14 i1="13"><s1>Office of the Director, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>24 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Bellini, William J" sort="Bellini, William J" uniqKey="Bellini W" first="William J." last="Bellini">William J. Bellini</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Respiratory and Enteric Virus Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>18 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Anderson, Larry J" sort="Anderson, Larry J" uniqKey="Anderson L" first="Larry J." last="Anderson">Larry J. Anderson</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Respiratory and Enteric Virus Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>18 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">03-0295548</idno><date when="2003">2003</date><idno type="stanalyst">PASCAL 03-0295548 INIST</idno><idno type="RBID">Pascal:03-0295548</idno><idno type="wicri:Area/PascalFrancis/Corpus">000983</idno><idno type="wicri:Area/PascalFrancis/Curation">000008</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000984</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000984</idno><idno type="wicri:doubleKey">0028-4793:2003:Ksiazek T:a:novel:coronavirus</idno><idno type="wicri:Area/Main/Merge">006933</idno><idno type="wicri:Area/Main/Curation">006421</idno><idno type="wicri:Area/Main/Exploration">006421</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">A novel coronavirus associated with severe acute respiratory syndrome</title><author><name sortKey="Ksiazek, Thomas G" sort="Ksiazek, Thomas G" uniqKey="Ksiazek T" first="Thomas G." last="Ksiazek">Thomas G. Ksiazek</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Special Pathogens Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Erdman, Dean" sort="Erdman, Dean" uniqKey="Erdman D" first="Dean" last="Erdman">Dean Erdman</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Respiratory and Enteric Virus Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>18 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Goldsmith, Cynthia S" sort="Goldsmith, Cynthia S" uniqKey="Goldsmith C" first="Cynthia S." last="Goldsmith">Cynthia S. Goldsmith</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Infectious Disease Pathology Activity, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Zaki, Sherif R" sort="Zaki, Sherif R" uniqKey="Zaki S" first="Sherif R." last="Zaki">Sherif R. Zaki</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Infectious Disease Pathology Activity, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Peret, Teresa" sort="Peret, Teresa" uniqKey="Peret T" first="Teresa" last="Peret">Teresa Peret</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Respiratory and Enteric Virus Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>18 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Emery, Shannon" sort="Emery, Shannon" uniqKey="Emery S" first="Shannon" last="Emery">Shannon Emery</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Respiratory and Enteric Virus Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>18 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Suxiang Tong" sort="Suxiang Tong" uniqKey="Suxiang Tong" last="Suxiang Tong">SUXIANG TONG</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Respiratory and Enteric Virus Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>18 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Urbani, Carlo" sort="Urbani, Carlo" uniqKey="Urbani C" first="Carlo" last="Urbani">Carlo Urbani</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>World Health Organization</s1><s2>Hanoi</s2><s3>VNM</s3><sZ>8 aut.</sZ></inist:fA14><country>Viêt Nam</country><wicri:noRegion>World Health Organization</wicri:noRegion></affiliation></author><author><name sortKey="Comer, James A" sort="Comer, James A" uniqKey="Comer J" first="James A" last="Comer">James A. Comer</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Special Pathogens Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Wilina Lim" sort="Wilina Lim" uniqKey="Wilina Lim" last="Wilina Lim">WILINA LIM</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Government Virus Unit, Queen Mary Hospital</s1><s3>HKG</s3><sZ>10 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Government Virus Unit, Queen Mary Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Rollin, Pierre E" sort="Rollin, Pierre E" uniqKey="Rollin P" first="Pierre E." last="Rollin">Pierre E. Rollin</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Special Pathogens Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Dowell, Scott F" sort="Dowell, Scott F" uniqKey="Dowell S" first="Scott F." last="Dowell">Scott F. Dowell</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>International Emerging Infectious Diseases Program</s1><s2>Bangkok</s2><s3>THA</s3><sZ>12 aut.</sZ></inist:fA14><country>Thaïlande</country><wicri:noRegion>International Emerging Infectious Diseases Program</wicri:noRegion></affiliation></author><author><name sortKey="Ling, Ai Ee" sort="Ling, Ai Ee" uniqKey="Ling A" first="Ai-Ee" last="Ling">Ai-Ee Ling</name><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Pathology, Singapore General Hospital</s1><s3>SGP</s3><sZ>13 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Department of Pathology, Singapore General Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Humphrey, Charles D" sort="Humphrey, Charles D" uniqKey="Humphrey C" first="Charles D." last="Humphrey">Charles D. Humphrey</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Infectious Disease Pathology Activity, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Shieh, Wun Ju" sort="Shieh, Wun Ju" uniqKey="Shieh W" first="Wun-Ju" last="Shieh">Wun-Ju Shieh</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Infectious Disease Pathology Activity, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Guarner, Jeannette" sort="Guarner, Jeannette" uniqKey="Guarner J" first="Jeannette" last="Guarner">Jeannette Guarner</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Infectious Disease Pathology Activity, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Paddock, Christopher D" sort="Paddock, Christopher D" uniqKey="Paddock C" first="Christopher D." last="Paddock">Christopher D. Paddock</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Infectious Disease Pathology Activity, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>17 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Rota, Paul" sort="Rota, Paul" uniqKey="Rota P" first="Paul" last="Rota">Paul Rota</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Respiratory and Enteric Virus Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>18 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Fields, Barry" sort="Fields, Barry" uniqKey="Fields B" first="Barry" last="Fields">Barry Fields</name><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>19 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Derisi, Joseph" sort="Derisi, Joseph" uniqKey="Derisi J" first="Joseph" last="Derisi">Joseph Derisi</name><affiliation wicri:level="3"><inist:fA14 i1="09"><s1>University of California</s1><s2>San Francisco</s2><s3>USA</s3><sZ>20 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><settlement type="city">San Francisco</settlement><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Yang, Jyh Yuan" sort="Yang, Jyh Yuan" uniqKey="Yang J" first="Jyh-Yuan" last="Yang">Jyh-Yuan Yang</name><affiliation wicri:level="1"><inist:fA14 i1="10"><s1>Center for Disease Control, Department of Health</s1><s2>Taipei</s2><s3>TWN</s3><sZ>21 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Cox, Nancy" sort="Cox, Nancy" uniqKey="Cox N" first="Nancy" last="Cox">Nancy Cox</name><affiliation wicri:level="1"><inist:fA14 i1="11"><s1>Influenza Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>22 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Hughes, James M" sort="Hughes, James M" uniqKey="Hughes J" first="James M." last="Hughes">James M. Hughes</name><affiliation wicri:level="1"><inist:fA14 i1="12"><s1>National Center for Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>23 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Leduc, James W" sort="Leduc, James W" uniqKey="Leduc J" first="James W." last="Leduc">James W. Leduc</name><affiliation wicri:level="1"><inist:fA14 i1="13"><s1>Office of the Director, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>24 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Bellini, William J" sort="Bellini, William J" uniqKey="Bellini W" first="William J." last="Bellini">William J. Bellini</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Respiratory and Enteric Virus Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>18 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author><author><name sortKey="Anderson, Larry J" sort="Anderson, Larry J" uniqKey="Anderson L" first="Larry J." last="Anderson">Larry J. Anderson</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Respiratory and Enteric Virus Branch, National Center for Infectious Diseases, Center for Disease Control and Prevention</s1><s2>Atlanta</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>18 aut.</sZ><sZ>25 aut.</sZ><sZ>26 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Atlanta</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">The New England journal of medicine</title><title level="j" type="abbreviated">N. Engl. j. med.</title><idno type="ISSN">0028-4793</idno><imprint><date when="2003">2003</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">The New England journal of medicine</title><title level="j" type="abbreviated">N. Engl. j. med.</title><idno type="ISSN">0028-4793</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult respiratory distress syndrome</term><term>Asia</term><term>Coronavirus</term><term>Human</term><term>Lung disease</term><term>Microbiological investigation</term><term>Mutation</term><term>Severe acute respiratory syndrome</term><term>Viral disease</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Poumon pathologie</term><term>Détresse respiratoire adulte syndrome</term><term>Virose</term><term>Homme</term><term>Coronavirus</term><term>Exploration microbiologique</term><term>Asie</term><term>Mutation</term><term>SRAS</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">BACKGROUND A worldwide outbreak of severe acute respiratory syndrome (SARS) has been associated with exposures originating from a single ill health care worker from Guangdong Province, China. We conducted studies to identify the etiologic agent of this outbreak. METHODS We received clinical specimens from patients in seven countries and tested them, using virus-isolation techniques, electron-microscopical and histologic studies, and molecular and serologic assays, in an attempt to identify a wide range of potential pathogens. RESULTS None of the previously described respiratory pathogens were consistently identified. However, a novel coronavirus was isolated from patients who met the case definition of SARS. Cytopathological features were noted in Vero E6 cells inoculated with a throatswab specimen. Electron-microscopical examination revealed ultrastructural features characteristic ofcoronaviruses. Immunohistochemical and immunofluorescence staining revealed reactivity with group I coronavirus polyclonal antibodies. Consensus coronavirus primers designed to amplify a fragment ofthe polymerase gene by reverse transcription-polymerase chain reaction (RT-PCR) were used to obtain a sequence that clearly identified the isolate as a unique coronavirus only distantly related to previously sequenced coronaviruses. With specific diagnostic RT-PCR primers we identified several identical nucleotide sequences in 12 patients from several locations, a finding consistent with a point-source outbreak. Indirect fluorescence antibody tests and enzyme-linked immunosorbent assays made with the new isolate have been used to demonstrate a virus-specific serologic response. This virus may never before have circulated in the U.S. population. CONCLUSIONS A novel coronavirus is associated with this outbreak, and the evidence indicates that this virus has an etiologic role in SARS. Because ofthe death ofDr. Carlo Urbani, we propose that our first isolate be named the Urbani strain of SARS-associated coronavirus.</div></front></TEI><affiliations><list><country><li>Hong Kong</li><li>Singapour</li><li>Taïwan</li><li>Thaïlande</li><li>Viêt Nam</li><li>États-Unis</li></country><region><li>Californie</li></region><settlement><li>San Francisco</li></settlement></list><tree><country name="États-Unis"><noRegion><name sortKey="Ksiazek, Thomas G" sort="Ksiazek, Thomas G" uniqKey="Ksiazek T" first="Thomas G." last="Ksiazek">Thomas G. Ksiazek</name></noRegion><name sortKey="Anderson, Larry J" sort="Anderson, Larry J" uniqKey="Anderson L" first="Larry J." last="Anderson">Larry J. Anderson</name><name sortKey="Bellini, William J" sort="Bellini, William J" uniqKey="Bellini W" first="William J." last="Bellini">William J. Bellini</name><name sortKey="Comer, James A" sort="Comer, James A" uniqKey="Comer J" first="James A" last="Comer">James A. Comer</name><name sortKey="Cox, Nancy" sort="Cox, Nancy" uniqKey="Cox N" first="Nancy" last="Cox">Nancy Cox</name><name sortKey="Derisi, Joseph" sort="Derisi, Joseph" uniqKey="Derisi J" first="Joseph" last="Derisi">Joseph Derisi</name><name sortKey="Emery, Shannon" sort="Emery, Shannon" uniqKey="Emery S" first="Shannon" last="Emery">Shannon Emery</name><name sortKey="Erdman, Dean" sort="Erdman, Dean" uniqKey="Erdman D" first="Dean" last="Erdman">Dean Erdman</name><name sortKey="Fields, Barry" sort="Fields, Barry" uniqKey="Fields B" first="Barry" last="Fields">Barry Fields</name><name sortKey="Goldsmith, Cynthia S" sort="Goldsmith, Cynthia S" uniqKey="Goldsmith C" first="Cynthia S." last="Goldsmith">Cynthia S. Goldsmith</name><name sortKey="Guarner, Jeannette" sort="Guarner, Jeannette" uniqKey="Guarner J" first="Jeannette" last="Guarner">Jeannette Guarner</name><name sortKey="Hughes, James M" sort="Hughes, James M" uniqKey="Hughes J" first="James M." last="Hughes">James M. Hughes</name><name sortKey="Humphrey, Charles D" sort="Humphrey, Charles D" uniqKey="Humphrey C" first="Charles D." last="Humphrey">Charles D. Humphrey</name><name sortKey="Leduc, James W" sort="Leduc, James W" uniqKey="Leduc J" first="James W." last="Leduc">James W. Leduc</name><name sortKey="Paddock, Christopher D" sort="Paddock, Christopher D" uniqKey="Paddock C" first="Christopher D." last="Paddock">Christopher D. Paddock</name><name sortKey="Peret, Teresa" sort="Peret, Teresa" uniqKey="Peret T" first="Teresa" last="Peret">Teresa Peret</name><name sortKey="Rollin, Pierre E" sort="Rollin, Pierre E" uniqKey="Rollin P" first="Pierre E." last="Rollin">Pierre E. Rollin</name><name sortKey="Rota, Paul" sort="Rota, Paul" uniqKey="Rota P" first="Paul" last="Rota">Paul Rota</name><name sortKey="Shieh, Wun Ju" sort="Shieh, Wun Ju" uniqKey="Shieh W" first="Wun-Ju" last="Shieh">Wun-Ju Shieh</name><name sortKey="Suxiang Tong" sort="Suxiang Tong" uniqKey="Suxiang Tong" last="Suxiang Tong">SUXIANG TONG</name><name sortKey="Zaki, Sherif R" sort="Zaki, Sherif R" uniqKey="Zaki S" first="Sherif R." last="Zaki">Sherif R. Zaki</name></country><country name="Viêt Nam"><noRegion><name sortKey="Urbani, Carlo" sort="Urbani, Carlo" uniqKey="Urbani C" first="Carlo" last="Urbani">Carlo Urbani</name></noRegion></country><country name="Hong Kong"><noRegion><name sortKey="Wilina Lim" sort="Wilina Lim" uniqKey="Wilina Lim" last="Wilina Lim">WILINA LIM</name></noRegion></country><country name="Thaïlande"><noRegion><name sortKey="Dowell, Scott F" sort="Dowell, Scott F" uniqKey="Dowell S" first="Scott F." last="Dowell">Scott F. Dowell</name></noRegion></country><country name="Singapour"><noRegion><name sortKey="Ling, Ai Ee" sort="Ling, Ai Ee" uniqKey="Ling A" first="Ai-Ee" last="Ling">Ai-Ee Ling</name></noRegion></country><country name="Taïwan"><noRegion><name sortKey="Yang, Jyh Yuan" sort="Yang, Jyh Yuan" uniqKey="Yang J" first="Jyh-Yuan" last="Yang">Jyh-Yuan Yang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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